Very little of Mark Twain National Forest is included in the $60 million acres of land under new federal protection. Charlotte Wiggins, spokeswoman for the U.S. Forest Service in Rolla, says lands out West near fast-developing areas were the target of the order, which prohibits new road construction. Wiggins says about 25,000 acres in Missouri are covered. They include 2,500 acres near Fredericktown in southeast Missouri, a few thousand acres around Willow Springs in south-central Missouri, and a few thousand close to Ava, southeast of Springfield.